TitleComparative expression profiling reveals an essential role for raldh2 in epimorphic regeneration.
Publication TypeJournal Article
Year of Publication2009
AuthorsMathew, LK, Sengupta, S, Franzosa, JA, Perry, J, La Du, J, Andreasen, EA, Tanguay, RL
JournalJ Biol Chem
Date Published2009 Nov 27
KeywordsAnimals, Butadienes, Cluster Analysis, Embryo, Nonmammalian, Extremities, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, In Situ Hybridization, Larva, Male, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Nitriles, Oligonucleotide Array Sequence Analysis, Pyrroles, Receptor, Fibroblast Growth Factor, Type 1, Regeneration, Retinal Dehydrogenase, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Wnt Proteins, Wound Healing, Zebrafish, Zebrafish Proteins

Zebrafish have the remarkable ability to regenerate body parts including the heart and fins by a process referred to as epimorphic regeneration. Recent studies have illustrated that similar to adult zebrafish, early life stage larvae also possess the ability to regenerate the caudal fin. A comparative microarray analysis was used to determine the degree of conservation in gene expression among the regenerating adult caudal fin, adult heart, and larval fin. Results indicate that these tissues respond to amputation/injury with strikingly similar genomic responses. Comparative analysis revealed raldh2, a rate-limiting enzyme for the synthesis of retinoic acid, as one of the most highly induced genes across the three regeneration platforms. In situ localization and functional studies indicate that raldh2 expression is critical for the formation of wound epithelium and blastema. Patterning during regenerative outgrowth was considered to be the primary function of retinoic acid signaling; however, our results suggest that it is also required for early stages of tissue regeneration. Expression of raldh2 is regulated by Wnt and fibroblast growth factor/ERK signaling.

Alternate JournalJ. Biol. Chem.
PubMed ID19801676
PubMed Central IDPMC2785206
Grant ListP40 RR012546 / RR / NCRR NIH HHS / United States
ES00210 / ES / NIEHS NIH HHS / United States
P30 ES003850 / ES / NIEHS NIH HHS / United States
P40 RR12546 / RR / NCRR NIH HHS / United States
ES03850 / ES / NIEHS NIH HHS / United States
ES10820 / ES / NIEHS NIH HHS / United States
R01 ES010820 / ES / NIEHS NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States